Endocrine Abstracts

Proadrenomedullin N-terminal 20 peptide (PAMP) and adrenomedullin are hypotensive peptides that are cleaved from the proadrenomedullin peptide. Adrenomedullin can act via the complex calcitonin receptor like receptor (CRLR) and receptor activity modifying protein (RAMP) 2 or the putative receptor, CRLR/RAMP3 complex. CGRP (Calcitonin gene related peptide) binding is conferred by the CRLR/RAMP1 complex. This study was carried out to see how adrenomedullin and PAMP affected the ...

THE REGULATION OF ADRENOMEDULLIN AND PROADRENOMEDULLIN N-TERMINAL 20 PEPTIDE IN THE ADRENAL GLAND: THE DIFFERENCE BETWEEN ADRENOCORTICAL CELLS AND CHROMAFFIN CELLS.Vakharia K, Hinson JP.Department of Endocrinology, Lab 1.2 Dominion House, Queen Mary University of London, London E, UK.It has been established that the adrenomedullin gene is expressed in the adrenal cortex and adrenal medulla, and that both adre...

Previous data from this laboratory has shown that lipopolysaccharide (LPS) is a direct stimulant of adrenal cortisol secretion. The present study was designed to determine the mechanism of this effect.The human adrenocortical H295R cell line was used in these studies. Cells were incubated in the presence of varying concentrations of LPS (10 to 1000ng/ml) for periods up to 24 hours. PGE2 and cortisol were measured by RIA. Indomethacin and the specific COX...

Adrenomedullin and pro-adrenomedullin N terminal 20 peptide (PAMP) are multi-functional peptides that are cleaved from a precursor peptide named proadrenomedullin. It has been well documented that adrenomedullin has an effect on cell growth and differentiation in many cell types. However, the effects of PAMP on cell growth still remain to be elucidated.Using the human endothelial cell line (HMEC-1) and human adrenocortical cell line (H295R), cells were p...

Bacterial Lipopolysaccharide directly stimulates Cortisol Secretion by Human Adrenal CellsK. Vakharia, D Renshaw, J.P. HinsonDepartment of Endocrinology, Division of General and Developmental Medicine, Barts and the London, Queen Mary School of Medicine and Dentistry, London, UK.Adrenomedullin (ADM) is a potent vasorelaxant peptide recently identified in extracts of pheochromocytoma. Proadrenomedullin, a pre...

Dehydroepiandrosterone sulphate is a steroid product of the adrenal gland, which circulates in micromolar concentrations, but whose function is unclear. Epidemiological studies have suggested that high circulating DHEAS levels may be protective against cardiovascular disease. The present study was designed to determine whether DHEAS altered vascular cell growth using the human microvascular endothelial cell line HMEC.HMECs were grown in MCDB131 medium co...

The factors that establish and maintain adrenocortical zonation and proliferation are poorly understood but include the pituitary hormone adrenocorticotrophin (ACTH), angiotensin II and potassium. To examine this capsular gland preparations largely glomerulosa (zg) with some fasciculata (zf), were cultured in vitro in Eagles MEM (3.6mM K+) for 1, 4 and 8 days and exposed these treatments. Rat zona glomerulosa proliferative activity, as judged by the rate of DNA synthesis, was ...